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Diclofenac ratiopharm 25 mg rezeptfrei (5 mg/kg, i.p.) or vehicle on the fourth day of experiment. animals were killed at the indicated times. Liver, spleen and gonad weights, cholesterol triglyceride levels were measured by gas chromatography. Blood glucose levels were measured by a glucometer attached to blood sampling device (Leica Microsystems Inc, St Louis, MO). The rats were housed in a climate-controlled, 12 h/dark cycle at 23 ± 1°C. On the last day of each experiment the animals were killed and liver was removed weighed; the weights of livers and spleens all male female rats were measured. The animals housed under controlled conditions and given food water ad libitum. Results The results indicate that 2-AG, which was present in the first 7 hr of dark cycle, is present in the serum of both female and male rats. The concentrations that we found in the serum of female rats were slightly higher than we found in the serum of male rats. As the concentrations of 2-AG in plasma female rats were not different from the concentrations found in plasma of male rats, we conclude that both female and male rats have 2-AG in their serum. DISCUSSION One of the main purposes this study is to determine the degree which 2-AG is present in the serum of male and female rats. It now appears that 2-AG is present in both male and female rats. In a previous study which rats were fed a normal diet or containing 0.5% (wt/wt) AAS, there was no difference in body weights of male and female rats when the AAS was stopped after 14 days (13). There was no difference in the weight gain of male rats fed 0.5% (wt/wt) AAS compared with the weight gain of male rats fed a diet containing 10% (wt/wt) AAS (13). The effects of on plasma levels 2-AG were similar at both doses but not the higher dose (13). The results of this study are consistent with our earlier findings in the rat that AAS did not affect the plasma concentrations of 2-AG in male or female rats (13, 14). The concentration of 2-AG in male rat was relatively higher than that in the female rat. There was a concentration gradient between the plasma and liver of male rats, both the plasma and liver of male female rats had concentrations higher than the plasma of female rats. male rats was slightly higher than the plasma of female rats. male rats was 1.5- to 2.0-fold higher than that of female rats. Although there was no difference in the plasma concentration of 2-AG in male and female rats, there was a concentration gradient in the plasma of male and female rats, both the plasma and liver of male female rats had concentrations higher than the plasma of female rats. Because the concentration gradient, some of compounds responsible for the difference in plasma concentrations of the 2-AG in male and female rats were more highly concentrated in the male and female rats. concentrations of 2-AG found in the plasma of male rats were 3 to 4 or more times higher than those found in the plasma of female rats. This was true for both the 10% (wt/wt) and 0.5% AAS groups. It is important to note that the concentrations of 2-AG in plasma female rats were similar to or lower than those of the plasma male rats. differences between the plasma levels of 2-AG in male and female rats were similar for both the 10% (wt/wt) and 0.5% AAS groups. AAS is known to have a variety of physiological effects in many organs vivo. animal models, some of these effects are not seen after AAS administration only. It has been shown that the AAS caused a decrease in the plasma levels of some steroid hormones such as testosterone, DHT, estradiol, LH, and FSH (2, 5, 14). However, many of the effects were seen in only a very few individuals. Another effect of AAS is that some these can also have an effect on some of the other steroid hormones such as estradiol (15). The results of present study indicate that there is a concentration gradient in the plasma of male and female rats that some of the compounds causing diclofenac creme rezeptfrei AAS to have an effect on plasma levels of the steroid hormones are present in the plasma of both male and female rats. The concentration gradient in plasma of both male and female rats was not seen with the 10% diclofenac sodium 50mg capsule (wt/wt) AAS, and concentrations were higher in the plasma of.

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